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BOR - Papers in Press, published online ahead of print May 14, 2008.
Biol Reprod 2008, 10.1095/biolreprod.107.066357
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BIOLOGY OF REPRODUCTION 79, 367–375 (2008)
DOI: 10.1095/biolreprod.107.066357
© 2008 by the Society for the Study of Reproduction, Inc.

research-article

Sperm Mitochondrial Integrity Is Not Required for Hyperactivated Motility, Zona Binding, or Acrosome Reaction in the Rhesus Macaque1

Pei-hsuan Hung 3 6, Marion G. Miller 4, Stuart A. Meyers 5, and Catherine A. VandeVoort 2 3 6

Molecular, Cellular, and Integrative Physiology,3 Pharmacology and Toxicology,4 Department of Anatomy, Physiology, and Cell Biology,5 and California National Primate Research Center,6 University of California, Davis, Davis, California 95616

ABSTRACT

Whether the main energy source for sperm motility is from oxidative phosphorylation or glycolysis has been long-debated in the field of reproductive biology. Using the rhesus monkey as a model, we examined the role of glycolysis and oxidative phosphorylation in sperm function by using alpha-chlorohydrin (ACH), a glycolysis inhibitor, and pentachlorophenol (PCP), an oxidative phosphorylation uncoupler. Sperm treated with ACH showed no change in percentage of motile sperm, although sperm motion was impaired. The ACH-treated sperm did not display either hyperactivity- or hyperactivation-associated changes in protein tyrosine phosphorylation. When treated with PCP, sperm motion parameters were affected by the highest level of PCP (200 µM); however, PCP did not cause motility impairments even after chemical activation. Sperm treated with PCP were able to display hyperactivity and tyrosine phosphorylation after chemical activation. In contrast with motility measurements, treatment with either the glycolytic inhibitor or the oxidative phosphorylation inhibitor did not affect sperm-zona binding and zona-induced acrosome reaction. The results suggest glycolysis is essential to support sperm motility, hyperactivity, and protein tyrosine phosphorylation, while energy from oxidative phosphorylation is not necessary for hyperactivated sperm motility, tyrosine phosphorylation, sperm-zona binding, and acrosome reaction in the rhesus macaque.

acrosome reaction, sperm, sperm capacitation

FOOTNOTES

1Supported by Philip Morris External Research Program, NIH grant RR00169, and by R01 RR016581.

Correspondence: 2Catherine A. VandeVoort, California National Primate Research Center, Roads 98 and Hutchison, University of California, Davis, CA 95616. FAX: 530 752 2880; e-mail: cavandevoort{at}ucdavis.edu






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Copyright © 2008 by the Society for the Study of Reproduction.