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BOR - Papers in Press, published online ahead of print January 23, 2008.
Biol Reprod 2008, 10.1095/biolreprod.107.066258
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BIOLOGY OF REPRODUCTION 79, 66–74 (2008)
DOI: 10.1095/biolreprod.107.066258
© 2008 by the Society for the Study of Reproduction, Inc.

research-article

From Hepatopancreas to Ovary: Molecular Characterization of a Shrimp Vitellogenin Receptor Involved in the Processing of Vitellogenin1

Shirley Hiu Kwan Tiu 3, John Benzie 4, and Siu-Ming Chan 2 3

School of Biological Sciences,3 The University of Hong Kong, Hong Kong, People's Republic of China Moana Technologies, LLC,4 Kailua Kona, Hawaii 96740

ABSTRACT

We report the first cloning and characterization of cDNA encoding a putative vitellogenin (Vg) receptor (VgR) from the shrimp, Penaeus monodon. The shrimp VgR cDNA is 6.8 kb; the deduced protein has 1943 amino acids with a molecular weight of 211 kDa. VgR is ovary specific and consists of conserved cysteine-rich domains, epidermal growth factor-like domains, and YWTD motifs similar to the low-density lipoprotein, very low-density lipoprotein, and VgR of insects and vertebrates. VgR expression level in the ovary is low during early vitellogenesis and increases to maximum levels in females with a gonadosomatic index of 3–4, presumably when needed for receptor-mediated endocytosis during the rapid phase of extraovarian Vg production by the hepatopancreas. A peptide from the C-terminal end of VgR was synthesized for antibody production. Anti-VgR antibody recognized an ovarian membrane protein, and the level of this protein was high when extraovarian production of Vg reached peak levels. By immunohistochemical analysis, VgR was detected strongly in the membranes of larger oocytes. VgR expression was knocked down after the shrimp were injected with VgR double-stranded RNA, leading to a decrease in VgR protein content in the ovary, but an increase in the hemolymph level of Vg. This study represents the first report of the functional analysis of a putative VgR in a crustacean.

endocytosis, gene regulation, oocyte development, ovary, receptor, receptor mediated endocytosis, vitellogenesis, vitellogenin, vitellogenin receptor

FOOTNOTES

1Supported by RGC grant HKU 7484/06M of the Hong Kong Special Administrative Region government to S.-M.C. The Penaeus monodon vitellogenin receptor cDNA sequence has been submitted to the GenBank database with accession number EU024890.

Correspondence: 2Siu-Ming Chan, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China. FAX: 852 2857 4672; e-mail: chansm{at}hkucc.hku.hk






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Copyright © 2008 by the Society for the Study of Reproduction.