Supplemental Figures -
"Supplemental Figure 1. Lysates (15 µg/lane) of quiescent sperm were separated by PAGE under non-reducing (left lane) or reducing (right lane) conditions and immunoblotted. The antibody to HK1 (H-95) detected the dimer band (arrow) in the non-reducing condition (-&bgr;-mercaptoethanol; BME) and monomer band in the reducing condition (+ BME).
Supplemental Figure 2. Lysates and pellets of sperm activated by incubating in M2 medium for 5 min (- capacitation) and of sperm incubated under capacitating conditions for 2 h (+ capacitation). Lysates and pellets from 0.5 × 107 sperm were separated by PAGE under reducing conditions and immunoblotted with antibodies to the SSR domain of HK1S, P-Tyr, and &agr;-tubulin (loading control).
Supplemental Figure 3. Sperm were treated with 0, 1, or 10 µM OA and motility (upper panel), HK activity (middle panel) and ATP levels (lower panel) determined in the presence of 0, 1, or 10 µM OA. Percent motility is indicated by the white bars and the percent progressive motility is indicated by black bars (upper panel). Data are expressed as the mean ± SD of three different experiments."
