Supplemental Data

Files in this Data Supplement:

• Supplemental Data - "Sequence alignment of SAMP32 protein in vertebrate species. SAMP32 peptide was searched for in NCBI (http://www.ncbi.nlm.nih.gov/) and found the SPACA1 gene. Using the NCBI link to Homologene (http://www.ncbi.nlm.nih.gov/sites/entrez/query.fcgi?db=homologene) the protein sequences of a number of vertebrate homologues were identified. A search of the ENSEMBL website (http://www.ensembl.org/index.html) with SPACA1 found a number of other homologs. A BLAST search of the non-mammalian EST dataset recovered two partial sequences from Elasmobranchii. The sequences were aligned using CLUSTAL (http://www.ebi.ac.uk/Tools/clustalw/index.html). Submission of the human SPACA1 peptide to SMART (http://smart.embl-heidelberg.de/) identified two conserved motifs, a putative signal peptide and a single transmembrane helix. Close examination of the CLUSTAL multiple alignment revealed 8 highly conserved cysteines. The EMBOSS program FUZZPRO was used to search for proteins containing a similar pattern of cysteines and this was found in the product of the gene TMEM81. In addition the TMEM81 protein had a signal peptide and a transmembrane helix. SMART suggested that the last two of the conserved cystines were part of an immunoglobulin domain. These, along with a conserved Tryptophan residue, allowed a similar Ig domain to be suggested in the SPACA1 sequence."