Supplemental Figures

Files in this Data Supplement:

• Supplemental Figure 2 - "Immunolocalization of DLX3 in cross-sections of filamentous ovine conceptuses at Day 15 of pregnancy. A, C) Sections were incubated with anti-DLX3. B, D) Control sections were incubated with normal rabbit serum. Bar = 200 µm (A, B) and 50 µm (C, D)."
• Supplemental Figure 1 - "Control images for immunocytochemistry in JAr cells (A-C) and CT-1 cells (D-F). JAr cells were probed with fluorescein (Alexa Fluor (AF) 488) labeled donkey anti-sheep (A), goat anti-rabbit (AF 568) (B), and DAPI (C). CT-1 cells were probed with fluorescein (AF 488)-labeled goat anti-mouse (D), goat anti-rabbit (AF 568) (E) and TO-PRO-3 iodide (F). All the experimental conditions, i.e. cell fixation, reagent concentrations, incubation times and image capture standards, were identical to the experimental conditions employed in Figure 6. Bar = 100 µm."
• Supplemental Figure 3 - "Association of endogenous ETS2 with the regulatory region actively transcribed IFNT from bovine CT-1 cells. Sheared chromatin prepared from formaldehyde-fixed CT-1 cells was analyzed by ChIP. Chromatin was exposed to no antibody (lane 1), anti-DLX3 (lane 2), anti-ETS2 (lane 3), purified rabbit IgG (lane 4), and anti-RNA polymerase II (lane 5). The DNA recovered from each immunocomplex was amplified with primers specific for a region (-188 to +3) containing the ETS2/DLX3 enhancer region within the IFNT promoter. Lane 6 represents the PCR product amplified from 10% of the total input chromatin. Lane 7 shows the PCR product amplified from 0.6 ng of plasmid DNA containing the -457luc reporter construct."