Supplemental Data -
"Supplemental TABLE S1. list of primers used for the RT-PCR and real-time PCR. The PCR products were also used as RNA probes for the in situ hybridization.
Supplement Figure S1. Alignment of trout (t) Bcmo1, Bcdo2, Rbp1, Lrat and Stra6 sequences with zebrafish (zf) and human (h) proteins are shown in A, B, C, D and E, respectively. The sequences for the trout proteins were obtained by computational translation of trout ESTs. The EST sequences for bcmo1, bcdo2, rbp1 and lrat were confirmed by sequencing PCR products. The sequence for stra6 was obtained as described in the Materials and Methods. Black lines under the trout sequences represent the location of the PCR primers. Black background represents identical residues. Gray background indicates conserved substitutions in the indicated species. Dots represent gaps introduced to maximize the alignment prepared with CLUSTALW program."
