The Birth of Mice from Testicular Spermatozoa Retrieved from Frozen Testicular Sections
Biol Reprod Ohta et al.
78: 807
Supplemental Figure
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Supplemental Figure -
"Male gamete preservation in "book" form. A, B) Frozen testicular sections from a C57BL/6 GFP transgenic mouse were mounted on a polypropylene sheet and placed in a photo album. C) Blastocysts at Embryonic Day 4.5 from oocytes fertilized by testicular spermatozoa retrieved from sections of A and B that had been frozen for 3 days at &[minus]30°C. D) Blastocysts featured in C exposed to the excitation light for GFP. Bars = 200 µm."
