Supplemental Figures -
"Supplemental Fig. 1. Detection of Kir6.2 (KCNJ11) and SUR2 (ABCC9) in the rat and mouse epididymis by the ABC-DAB technique. Negative controls. A, B) Rat epididymis (corpus), rabbit IgG (A), goat IgG (B). C, D) Mouse epididymis (corpus), rabbit IgG (C), goat IgG (D). Bar = 25 µm.
Supplemental Fig. 2. Detection of Kir6.2 (KCNJ11) and SUR2 (ABCC9) in the rat pancreas (control tissue) by the ABC-DAB technique. A, B) Positive controls: Kir6.2 (KCNJ11) (A) labels the endocrine islet cells; SUR2 (ABCC9) (B) labels the blood vessels. C, D) Negative controls: rabbit IgG (C), goat IgG (D). Bar = 25 µm.
Supplemental Fig. 3. Detection of Kir6.1 (KCNJ8) in the mouse epididymis by indirect immunofluorescence. Mouse epididymis (A, B), rat portal vein (positive control tissue) (C, D). Kir6.1 (KCNJ8) (A,C) and Rabbit IgG (negative controls) (B,D) (green). Bar = 25 µm.
Supplemental Fig. 4. Detection of SUR1 (ABCC8) in the rat epididymis by indirect immunofluorescence. Rat epididymis (A, B), rat pancreas (positive control tissue) (C, D). SUR1 (ABCC8) (A,C) and Goat IgG (negative controls) (B,D) (red). Bar = 25 µm.
Supplemental Fig. 5. Detection of Kir6.2 (KCNJ11) and SUR2 (ABCC9) in epididymides from various species by the ABC-DAB technique. A, C, E, G) Kir6.2 (KCNJ11). B, D, F, H) SUR2 (ABCC9). Canine (A, B), feline (C, D), bovine (E, F), human (G, H). Bar = 25 µm.
Supplemental Fig. 6. Detection of Kir6.2 (KCNJ11) and SUR2 (ABCC9) in epididymides from various species by indirect immunofluorescence. Negative controls. Canine (A; initial segment), feline (B; corpus), bovine epididymis (C; corpus), human (D; cauda). Overlay images of Rabbit IgG (green) and Goat IgG (red). Bar = 25 µm."
