Supplemental Data

Files in this Data Supplement:

• [Supplemental Figure 3] - "Proliferation of CD4⁺ and CD8⁺ T cell cultured on splenocytes. Freshly prepared-CFSE-labeled total T cells were plated with splenocytes for 5 days. CD4⁺ and CD8⁺ live-gated T cells were separately analyzed for their proliferation by CFSE staining. On each plot, the percentage of undivided cells is reported as a measure of the proliferation rate. Each trace is representative of triplicates samples within a single experiment of three independent experiments."
• [Supplemental Figure 1] - "PD-L2, B7-H4, B7-H3 and PD-L1 expression on mouse Sertoli cells treated with IFNG. Flow cytometric analysis was performed on Sertoli cell cultures, untreated (CTR) or with IFNG 500 U/ml) for 48 h. Cells were labeled with PE-conjugated specific antibodies. The grey area represents the isotypic control antibody fluorescence. Each trace is representative of triplicate samples within a single experiment of at least three independent experiments."
• [Supplemental Figure 2] - "B7-1 (CD80), B7-2 (CD86), CD40 expression on mouse Sertoli cells treated with IFNG. Flow cytometric analysis was performed on Sertoli cell cultures, untreated (CTR) or with IFNG 500 U/ml) for 48 h. Cells were labeled with PE-conjugated specific antibodies. The grey area represents the isotypic control antibody fluorescence. Each trace is representative of triplicate samples within a single experiment of at least three independent experiments."