Spatio-Temporal Expression Patterns of Aurora Kinases A, B, and C and Cytoplasmic Polyadenylation-Element-Binding Protein in Bovine Oocytes During Meiotic Maturation
Biol Reprod Uzbekova et al.
78: 218
Supplemental Data
Files in this Data Supplement:
[Supplemental Figure 2] -
"Immunoblot detection of the AURKA and phospho-MAPK1 during IVM in the presence or absence of 50 µM Roscovitine using the special extraction procedure [46]. Two shifted bands of AURKA were detected; the upper band might represent the phosphorylated form."
[Supplemental Figure 1] -
"Aurora kinases sequences analysis. A) Alignment of bovine Aurora kinases AURKA, AURKB, and AURKC amino-acid sequences. Underline delimits serine-threonine kinase catalytic domains. Catalytic sites are black-boxed. B) Comparison of bovine, human and mouse AURKA amino acid sequences (GenBank accession numbers: bovine ABC61056, human O14965, mouse P97477). Identical amino acids are shaded. Putative APC/C recognition sequences A-box and D-boxes are outlined."