Supplemental Data

Files in this Data Supplement:

• [Supplemental Data 3] - "Alignment of the exons encoding the 6-cysteine arrays of SPAG11C, SPAG11D/E, DEFB103A and DEFB104B orthologs using the CLUSTALW program. This file was used to identify highly conserved, surface-localized residues that may be functionally and structurally important using the ConSeq server (http://conseq.bioinfo.tau.ac.il/). This alignment was also used for phylogenetic analyses showed in Fig. 5. GenBank accession numbers: SPAG11C: human NM_058203, macaque AY528234, bull DQ838981, pig BX925543, dog DQ012011, mouse AY552530, rat DQ012093; SPAG11D/E: human NM_058201, macaque AY528235, bull DQ838981, pig BK005523, dog DQ012012, mouse NM_153115, rat NM_145087; DEFB103: human NM_018661 (DEFB103A), bull AJ568025, pig AY460575, dog DQ011972; DEFB104: human BC100850 (DEFB104B), bull from the genomic NW_995797, dog DQ011973."
• [Supplemental Data 1] - "DNA fragment of the Bos taurus 27q1.2 chromosome (GenBank accession number CM000203) containing one copy of the SPAG11 gene. The gene is composed of 8 exons, spanning about 20 kb, with arrangements of exons and introns as indicated in Fig 2."
• [Supplemental Data 2B] - "Animated three-dimensional projections showing the bovine SPAG11C peptide model rotating. Model was created using PyMol (DeLano Scientific, LLC, San Carlos, CA) and assembled using VideoMach (www.gromada.com/videomach.html)."
• [Supplemental Data 2A] - "Animated three-dimensional projections showing the bovine SPAG11C peptide model rotating. Model was created using PyMol (DeLano Scientific, LLC, San Carlos, CA) and assembled using VideoMach (www.gromada.com/videomach.html)."