Transcriptome Analysis of Bull Semen with Extreme Nonreturn Rate: Use of Suppression-Subtractive Hybridization to Identify Functional Markers for Fertility
Biol Reprod Lalancette et al.
78: 618
Supplemental Data
Files in this Data Supplement:
[Supplemental Table 1] -
"Primers used for the verification of any genomic or RNA contaminants in sample."
[Supplemental Figure 1] -
"Verification by PCR of the absence of contaminants using CDH45 and MYC markers. A 100 ng equivalent of aRNA aliquot from the respective RT performed for each bull have been tested by PCR using the appropriate primers (Supplementary Table S1). Arbitrary name is give for each bull as indicated for the corresponding lane of the agarose gel electrophoresis. The 1Kb molecular weight ladder (M). The respective expected fragment length is indicate on each side of the gel, reporting PCR results of the CDH1 (A) and CD45 (B) markers."