Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Fig. 1. Methods for enucleation and sham-enucleation of porcine oocytes. Oocytes for enucleation (A, B, C) and sham-enucleation (D, E, F) were held with a holding pipettes (A, D), and their zonae pellucidae close to the GV (B) and apart from the GV (E) were cut with injection pipettes without damaging the oolemma, respectively. The oocytes were then aspirated by the holding pipettes, so that GV (C) and the equivalent volume of cytoplasm (F) were pushed out of the zonae through the slits. Arrowheads indicate the GVs. The removed karyoplast and the cytoplast are compared (G) and the presence and the absence of DNA are confirmed by Hoechst staining (H). Scale bars indicate 50 µm.
• Supplemental Figure 2 - MPF activities in sham-enucleated and denuded porcine oocytes. MPF activities in sham-enucleated and denuded porcine oocytes were assayed as histone H1 kinase activity. Experiments were repeated twice and led the almost same results. A typical example is shown.