Supplemental Data

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• Supplemental Figure 1 - The specificity of anti-SWC3a (CD172a) antibody for porcine peripheral mononuclear blood cells. Immunoreactivity of antibodies was analyzed by flow cytometry. Mouse anti-porcine wCD8a antibody (UK-Serotec Ltd., Oxford, UK) and mouse anti-porcine CD3 antibody (VMRD, Inc., Pullman, WA) were used as T cell marker. Specific binding was detected with FITC- and PerCP-labeled secondary antibodies. The staining intensity of the cells is shown as dot plots. A) Immunoreaction with antibodies for SWC3a and CD8. (a) and (d) indicate CD172a- and CD8-positive cells, respectively. B) Immunoreaction with antibodies for CD3 and CD8. (a) and (d) indicate CD3- and CD8-specific positive cells, respectively, while (b) indicates both CD3- and CD8-positive cells. C) negative control. Note the no cross-reaction of anti-CD172a antibody with CD8 positive cells.