Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print January 11, 2006.
Biol Reprod 2006, 10.1095/biolreprod.105.047522
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
74/4/760    most recent
biolreprod.105.047522v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Caballero-Campo, P.
Right arrow Articles by Gerton, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caballero-Campo, P.
Right arrow Articles by Gerton, G. L.
Agricola
Right arrow Articles by Caballero-Campo, P.
Right arrow Articles by Gerton, G. L.
BIOLOGY OF REPRODUCTION 74, 760–768 (2006)
DOI: 10.1095/biolreprod.105.047522
© 2006 by the Society for the Study of Reproduction, Inc.

Research Article

Biological Effects of Recombinant Human Zona Pellucida Proteins on Sperm Function1

Pedro Caballero-Campo 3 4, Mayel Chirinos 3 5, Xue J. Fan 4, María E. González-González 5, Myrna Galicia-Chavarría 5, Fernando Larrea 5, and George L. Gerton 2 4

Center for Research on Reproduction and Women's Health,4 University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6080 Departamento de Biología de la Reproducción,5 Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14000, Mexico

ABSTRACT

The initial interaction between gametes takes place at the level of the sperm surface and the zona pellucida (ZP), the extracellular matrix of the egg in mammals. Successful fertilization requires the proper molecular recognition of the ZP by the sperm. Recently, human ZP was demonstrated to be composed of four proteins: ZP1, ZP2, ZP3, and ZP4. The goals of this study were to determine the effects of recombinant human ZP2, ZP3, and ZP4 on human sperm acrosomal exocytosis and sperm motility. Exposure of sperm to ZP proteins, alone or in combination, promoted acrosomal exocytosis in a time-dependent manner. This effect occurred in parallel with a considerable decrease in progressive motility, coincident with an increase in nonprogressive sperm motility. An analysis of kinetic parameters of ZP-treated sperm demonstrated that a characteristic motility pattern could be defined by values of curvilinear velocity > 63.9 µm/s and linearity ≤ 15.5%. A strong correlation between curvilinear velocity and the amplitude of lateral head displacement was also observed. The incidence of sperm having these particular kinetic parameters increased after exposure to ZP proteins. These studies of two processes involved in sperm penetration through the ZP confirm that zona glycoproteins promote acrosomal exocytosis and now establish an additional role for these components as modifiers of sperm motility.

acrosome reaction, acrosomal exocytosis, fertilization, gamete biology, sperm capacitation, sperm motility and transport, zona pellucida

FOOTNOTES

1 Supported by Contraceptive Research and Development (CONRAD) Twinning Program grants MFG-02–62 and MFG-02–63, NIH HD-41552, and the National Council of Science and Technology (CONACyT, Mexico). Also supported in part by the Bill and Melinda Gates Foundation (to X.J.F.). P.C.C. is a recipient of a training fellowship from Fundación Salud 2000 and Fundación Tambre, Madrid, Spain. M.E.G.G. and M.G.C. have been supported by CONACyT, Mexico.

2 Correspondence: George L. Gerton, Center for Research on Reproduction and Women's Health, Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, 421 Curie Blvd., 1311 BRB II/III, Philadelphia, PA 19104-6080. FAX: 215 573 7627; gerton{at}mail.med.upenn.edu

3 These authors contributed equally to this work.




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
P. C.N. Chiu, B. S.T. Wong, M.-K. Chung, K. K.W. Lam, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung
Effects of Native Human Zona Pellucida Glycoproteins 3 and 4 on Acrosome Reaction and Zona Pellucida Binding of Human Spermatozoa
Biol Reprod, November 1, 2008; 79(5): 869 - 877.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
P. C.N. Chiu, B. S.T. Wong, C.L. Lee, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung
Native human zona pellucida glycoproteins: purification and binding properties
Hum. Reprod., June 1, 2008; 23(6): 1385 - 1393.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Zhang, M. A Zariwala, M. M Mahadevan, P. Caballero-Campo, X. Shen, E. Escudier, B. Duriez, A.-M. Bridoux, M. Leigh, G. L Gerton, et al.
A Heterozygous Mutation Disrupting the SPAG16 Gene Results in Biochemical Instability of Central Apparatus Components of the Human Sperm Axoneme
Biol Reprod, November 1, 2007; 77(5): 864 - 871.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C.G. Ravina, M. Seda, F.M. Pinto, A. Orea, M. Fernandez-Sanchez, C.O. Pintado, and M.L. Candenas
A role for tachykinins in the regulation of human sperm motility
Hum. Reprod., June 1, 2007; 22(6): 1617 - 1625.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
Y. Ni, K. Li, W. Xu, L. Song, K. Yao, X. Zhang, H. Huang, Y. Zhang, and Q.-X. Shi
Acrosome Reaction Induced by Recombinant Human Zona Pellucida 3 Peptides rhuZP3a22~176 and rhuZP3b177~348 and Their Mechanism
J Androl, May 1, 2007; 28(3): 381 - 388.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Society for the Study of Reproduction.