Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hillier, S. G.
Right arrow Articles by Winston, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hillier, S. G.
Right arrow Articles by Winston, R. M.
Agricola
Right arrow Articles by Hillier, S. G.
Right arrow Articles by Winston, R. M.

Biology of Reproduction, Vol 31, 679-686, Copyright © 1984 by Society for the Study of Reproduction

ARTICLES

Granulosa cell steroidogenesis before in vitro fertilization

SG Hillier, EJ Wickings, M Afnan, RA Margara and RM Winston

Endocrine and gametogenic functions of the ovulatory follicle may be linked. To verify this, we studied granulosa cell steroidogenesis in relation to oocyte fertilization and preimplantation embryo development in vitro. Multiple follicles were stimulated in in vitro fertilization patients with clomiphene citrate and ovulation was induced with human chorionic gonadotropin (hCG). Oocytes were fertilized with husband's sperm and normal embryos were replaced 48 h later. Granulosa cells were separated from follicular fluid from 64 follicles and incubated for 3 h with and without aromatase substrate (1 microM testosterone). Progesterone and estradiol levels were measured in follicular fluid and incubation medium. Follicular fluid steroid levels and granulosa cell steroidogenesis showed no significant differences for oocytes which cleaved normally and those which did not. Granulosa cell aromatase activity was high in all follicles, suggesting that the low periovulatory follicular fluid estradiol level is not explained by a fall in granulosa cell aromatase after hCG. High granulosa cell progesterone production and follicular fluid progesterone were consistent with advanced granulosa cell luteinization. Oocytes undergoing polyspermic activation were from larger follicles with elevated follicular fluid progesterone levels, suggesting that follicular size and follicular fluid progesterone are correlated with "over-ripeness" and polyspermy. No simple relationship exists between oocyte function and the present indices of granulosa cell steroid metabolism.


This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
M.-G. Li, G.-L. Ding, X.-J. Chen, X.-P. Lu, L.-J. Dong, M.-Y. Dong, X.-F. Yang, X.-E Lu, and H.-F. Huang
Association of Serum and Follicular Fluid Leptin Concentrations with Granulosa Cell Phosphorylated Signal Transducer and Activator of Transcription 3 Expression in Fertile Patients with Polycystic Ovarian Syndrome
J. Clin. Endocrinol. Metab., December 1, 2007; 92(12): 4771 - 4776.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
P. Y. K. Yong, K. J. Thong, R. Andrew, B. R. Walker, and S. G. Hillier
Development-Related Increase in Cortisol Biosynthesis by Human Granulosa Cells
J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4728 - 4733.
[Abstract] [Full Text]

Home page
 Hum ReprodHome page
C. L. Chaffin, D. L. Hess, and R. L. Stouffer
Dynamics of periovulatory steroidogenesis in the rhesus monkey follicle after ovarian stimulation
Hum. Reprod., March 1, 1999; 14(3): 642 - 649.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the Society for the Study of Reproduction.