Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shivaji, S.
Right arrow Articles by Scheit, K. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shivaji, S.
Right arrow Articles by Scheit, K. H.
Agricola
Right arrow Articles by Shivaji, S.
Right arrow Articles by Scheit, K. H.

Biology of Reproduction, Vol 30, 1237-1241, Copyright © 1984 by Society for the Study of Reproduction

ARTICLES

Immunological identification of seminalplasmin in tissue extracts of sex glands of bull

S Shivaji, PM Bhargava and KH Scheit

Using immunoglobulin G (IgG) antibodies raised against highly purified, homogeneous seminalplasmin, an antimicrobial protein of bovine seminal plasma, it has been shown that bovine ampullae, gland vesicularis and corpus prostate, but not testes and epididymis, contain seminalplasmin. The content as estimated by radioimmunoassay employing 125I- seminalplasmin was: ampullae, 267 +/- 13; gland vesicularis, 275 +/- 14; and corpus prostate, 445 +/- 22 micrograms per g wet weight of the tissue. Seminalplasmin, as characterized by high-performance liquid chromatography and in vivo inhibition of RNA synthesis in E. coli, was isolated from gland vesicularis. The seminalplasmin content of bovine seminal plasma was shown to be 1%. A chymotryptic peptide of seminalplasmin comprising residues 1-13 from the amino terminus was found to compete with 125I-seminalplasmin for binding to anti- seminalplasmin IgG.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
M.A. Palladino, T.A. Mallonga, and M.S. Mishra
Messenger RNA (mRNA) Expression for the Antimicrobial Peptides {beta}-Defensin-1 and {beta}-Defensin-2 in the Male Rat Reproductive Tract: {beta}-Defensin-1 mRNA in Initial Segment and Caput Epididymidis Is Regulated by Androgens and Not Bacterial Lipopolysaccharides
Biol Reprod, February 1, 2003; 68(2): 509 - 515.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the Society for the Study of Reproduction.