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Biology of Reproduction, Vol 30, 687-691, Copyright © 1984 by Society for the Study of Reproduction

ARTICLES

Visualization of pronuclei in living bovine zygotes

BS Minhas, JS Capehart, MJ Bowen, JE Womack, JD McCrady, PG Harms, TE Wagner and DC Kraemer

Bovine embryos were surgically collected from the oviducts of superovulated crossbred heifers 48 h postonset of estrus. The one- celled ova were treated with 4'-6'-diamidino-2-phenylindole (DAPI) and observed under ultraviolet light by fluorescence microscopy. Both male and female pronuclei were visualized, identified and subjected to micromanipulation.




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Copyright © 1984 by the Society for the Study of Reproduction.