Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lalli, M. F.
Right arrow Articles by Clermont, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lalli, M. F.
Right arrow Articles by Clermont, Y.
Agricola
Right arrow Articles by Lalli, M. F.
Right arrow Articles by Clermont, Y.

Biology of Reproduction, Vol 30, 493-505, Copyright © 1984 by Society for the Study of Reproduction

ARTICLES

Glycoprotein synthesis in Sertoli cells during the cycle of the seminiferous epithelium of adult rats: a radioautographic study

MF Lalli, XM Tang and Y Clermont

Glycoprotein synthesis in Sertoli cells was investigated in rats sacrificed at various intervals (10, 30 min; 1, 4, 8, 24 h) after a single intratesticular injection of [3H] fucose. Thin sections of glutaraldehyde-fixed testes were radioautographed and the relative concentration of the label over the various cytoplasmic components of the Sertoli cells was analyzed quantitatively from electron microscopic photographs. The analysis was performed at the various stages of the cycle of the seminiferous epithelium grouped as follows: I-IV, V-VII, VIII-XI, XII-XIV. The incorporation of [3H] fucose into glycoproteins took place initially in the Golgi apparatus of the cells and the labeled glycoproteins later migrated to the lysosomes and plasma membrane. The corrected grain counts over the various cytoplasmic components of the Sertoli cell at various intervals after [3H] fucose injection, indicated that the synthesis of glycoproteins detected by radioautography after [3H] fucose injection was constant during the cycle of the seminiferous epithelium. Furthermore, the rate of transfer of the labeled glycoproteins from the Golgi apparatus to the lysosomes and plasma membrane was also noncyclic.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the Society for the Study of Reproduction.