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Biology of Reproduction, Vol 30, 199-211, Copyright © 1984 by Society for the Study of Reproduction
ARTICLES |
PS Tung, MK Skinner and IB Fritz
With indirect immunofluorescent microscopic techniques, we have shown that fibronectin is distributed primarily in or along the basal lamina of the seminiferous tubule boundary tissue in sections of testes from 20-day-old rats. Purified rat Sertoli cell-enriched aggregates, maintained in culture in the presence or absence of serum, exhibit no detectable immunofluorescence with fibronectin antibody, whereas purified peritubular cells in culture do have a positive reaction to fibronectin antibody. Peritubular cells in culture incorporate [35S] methionine into fibronectin which can be immunoprecipitated with a fibronectin antiserum, but Sertoli cells do not. We have used various criteria to estimate the degree of purity of Sertoli cell-enriched preparations. The presence of peritubular myoid cells in conventional Sertoli cell-enriched aggregates, cultured in the presence or absence of serum, can be detected with transmission electron microscopic examination, by the Feulgen staining procedure, and by the immunocytochemical identification of fibronectin. We describe a technique to purify Sertoli cells in conventional Sertoli cell-enriched preparations by treatment with hyaluronidase, resulting in a lesser number of peritubular cells by the above criteria, even in preparations cultured in the presence of serum. Data presented suggest that some of the products previously attributed exclusively to Sertoli cells in Sertoli cell-enriched preparations, particularly those cultured in the presence of serum, may have been contributed by peritubular cells.
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N. Bhattacharya, J. M. Dufour, M.-N. Vo, J. Okita, R. Okita, and K. H. Kim Differential Effects of Phthalates on the Testis and the Liver Biol Reprod, March 1, 2005; 72(3): 745 - 754. [Abstract] [Full Text] [PDF]
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 M. K. Y. Siu, D. D. Mruk, W. M. Lee, and C. Y. Cheng Adhering Junction Dynamics in the Testis Are Regulated by an Interplay of {beta}1-Integrin and Focal Adhesion Complex-Associated Proteins Endocrinology, May 1, 2003; 144(5): 2141 - 2163. [Abstract] [Full Text] [PDF]
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J. M. Dufour, M.-N. Vo, N. Bhattacharya, J. Okita, R. Okita, and K. H. Kim Peroxisome Proliferators Disrupt Retinoic Acid Receptor Alpha Signaling in the Testis Biol Reprod, April 1, 2003; 68(4): 1215 - 1224. [Abstract] [Full Text] [PDF]
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N. P.Y. Lee, D. Mruk, W. M. Lee, and C. Y. Cheng Is the Cadherin/Catenin Complex a Functional Unit of Cell-Cell Actin-Based Adherens Junctions in the Rat Testis? Biol Reprod, February 1, 2003; 68(2): 489 - 508. [Abstract] [Full Text] [PDF]
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M. K. Y. Siu, W. M. Lee, and C. Y. Cheng The Interplay of Collagen IV, Tumor Necrosis Factor-{alpha}, Gelatinase B (Matrix Metalloprotease-9), and Tissue Inhibitor of Metalloproteases-1 in the Basal Lamina Regulates Sertoli Cell-Tight Junction Dynamics in the Rat Testis Endocrinology, January 1, 2003; 144(1): 371 - 387. [Abstract] [Full Text] [PDF]
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K. W. Braun, M.-N. Vo, and K. H. Kim Positive Regulation of Retinoic Acid Receptor Alpha by Protein Kinase C and Mitogen-Activated Protein Kinase in Sertoli Cells Biol Reprod, July 1, 2002; 67(1): 29 - 37. [Abstract] [Full Text] [PDF]
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J. Chaudhary and M. K. Skinner Identification of a Novel Gene Product, Sertoli Cell Gene with a Zinc Finger Domain, That Is Important for FSH Activation of Testicular Sertoli Cells Endocrinology, February 1, 2002; 143(2): 426 - 435. [Abstract] [Full Text] [PDF]
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I. Goddard, S. Bauer, A. Gougeon, F. Lopez, N. Giannetti, C. Susini, M. Benahmed, and S. Krantic Somatostatin Inhibits Stem Cell Factor Messenger RNA Expression by Sertoli Cells and Stem Cell Factor-Induced DNA Synthesis in Isolated Seminiferous Tubules Biol Reprod, December 1, 2001; 65(6): 1732 - 1742. [Abstract] [Full Text] [PDF]
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J. Chaudhary and M. K. Skinner Role of the Transcriptional Coactivator CBP/p300 in Linking Basic Helix-Loop-Helix and CREB Responses for Follicle-Stimulating Hormone-Mediated Activation of the Transferrin Promoter in Sertoli Cells Biol Reprod, August 1, 2001; 65(2): 568 - 574. [Abstract] [Full Text] [PDF]
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J. Chaudhary, J. Johnson, G. Kim, and M. K. Skinner Hormonal Regulation and Differential Actions of the Helix-Loop-Helix Transcriptional Inhibitors of Differentiation (Id1, Id2, Id3, and Id4) in Sertoli Cells Endocrinology, May 1, 2001; 142(5): 1727 - 1736. [Abstract] [Full Text]
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L. Konrad, M. Albrecht, H. Renneberg, and G. Aumuller Transforming Growth Factor-{beta}2 Mediates Mesenchymal-Epithelial Interactions of Testicular Somatic Cells Endocrinology, October 1, 2000; 141(10): 3679 - 3686. [Abstract] [Full Text] [PDF]
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J. Chaudhary, R. Mosher, G. Kim, and M. K. Skinner Role of Winged Helix Transcription Factor (WIN) in the Regulation of Sertoli Cell Differentiated Functions: WIN Acts as an Early Event Gene for Follicle-Stimulating Hormone Endocrinology, August 1, 2000; 141(8): 2758 - 2766. [Abstract] [Full Text] [PDF]
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S. Krantic and M. Benahmed Somatostatin Inhibits Follicle-Stimulating Hormone-Induced Adenylyl Cyclase Activity and Proliferation in Immature Porcine Sertoli Cell via sst2 Receptor Biol Reprod, June 1, 2000; 62(6): 1835 - 1843. [Abstract] [Full Text]
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 J. Chaudhary and M. K. Skinner Basic Helix-Loop-Helix Proteins Can Act at the E-Box within the Serum Response Element of the c-fos Promoter to Influence Hormone-Induced Promoter Activation in Sertoli Cells Mol. Endocrinol., May 1, 1999; 13(5): 774 - 786. [Abstract] [Full Text]
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J. Chaudhary and M. K. Skinner E-Box and Cyclic Adenosine Monophosphate Response Elements Are Both Required for Follicle-Stimulating Hormone-Induced Transferrin Promoter Activation in Sertoli Cells Endocrinology, March 1, 1999; 140(3): 1262 - 1271. [Abstract] [Full Text]
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D. Nehar, C. Mauduit, F. Boussouar, and M. Benahmed Interleukin 1{alpha} Stimulates Lactate Dehydrogenase A Expression and Lactate Production in Cultured Porcine Sertoli Cells Biol Reprod, December 1, 1998; 59(6): 1425 - 1432. [Abstract] [Full Text]
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S. Ulisse, N. Rucci, D. Piersanti, E. Carosa, F. M. Graziano, A. Pavan, P. Ceddia, M. Arizzi, P. Muzi, L. Cironi, et al. Regulation by Thyroid Hormone of the Expression of Basement Membrane Components in Rat Prepubertal Sertoli Cells Endocrinology, February 1, 1998; 139(2): 741 - 747. [Abstract] [Full Text] [PDF]
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D. Nehar, C. Mauduit, F. Boussouar, and M. Benahmed Tumor Necrosis Factor-{alpha}-Stimulated Lactate Production Is Linked to Lactate Dehydrogenase A Expression and Activity Increase in Porcine Cultured Sertoli Cells Endocrinology, May 1, 1997; 138(5): 1964 - 1971. [Abstract] [Full Text] [PDF]
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J. Chaudhary, A. S. Cupp, and M. K. Skinner Role of Basic-Helix-Loop-Helix Transcription Factors in Sertoli Cell Differentiation: Identification of an E-Box Response Element in the Transferrin Promoter Endocrinology, February 1, 1997; 138(2): 667 - 675. [Abstract] [Full Text] [PDF]
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