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Biology of Reproduction, Vol 29, 1172-1178, Copyright © 1983 by Society for the Study of Reproduction

ARTICLES

Studies with purified immature porcine Leydig cells in primary culture

M Bernier, W Gibb, F Haour, R Collu, JM Saez and JR Ducharme

The steroidogenic capacity of purified immature porcine Leydig cells in culture was studied over several days. The cells were obtained by fractionating crude testicular interstitial cell suspensions on a discontinuous Percoll gradient (d = 1.037, 1.042, 1.052, 1.098 g/ml), and characterized by specific binding of 125I-human chorionic gonadotropin (hCG), testosterone (T) and cyclic adenosine 3':5'- monophosphate (cAMP) production in response to hCG, and the enzymatic determination of delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta- HSD) activity. The Leydig cells were recovered in a density band between 1.052-1.068 g/ml and grown in a chemically defined medium (Mather et al., 1981). In the absence of hCG, T production was low throughout the 6 days of culture. However, in response to hCG (10 mIU/ml), the cultured Leydig cells showed a progressive increase in T synthesis, which reached a maximum at Days 3-4. 8-Br-cAMP (1 mM) induced a comparable rise in T production to that obtained with hCG throughout the culture period. In contrast, 8-Br-cAMP induced a near maximal increase in dehydroepiandrosterone (DHEA) production from Day 1. This paper demonstrates that purified immature porcine Leydig cells in primary culture are a valuable model to study the ontogeny of Leydig cell function.


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F. M. Moran, J. J. Ford, C. J. Corbin, S. M. Mapes, V. C. Njar, A. M. Brodie, and A. J. Conley
Regulation of Microsomal P450, Redox Partner Proteins, and Steroidogenesis in the Developing Testes of the Neonatal Pig
Endocrinology, September 1, 2002; 143(9): 3361 - 3369.
[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the Society for the Study of Reproduction.