Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MacLaughlin, D. T.
Right arrow Articles by Richardson, G. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MacLaughlin, D. T.
Right arrow Articles by Richardson, G. S.
Agricola
Right arrow Articles by MacLaughlin, D. T.
Right arrow Articles by Richardson, G. S.

Biology of Reproduction, Vol 29, 733-742, Copyright © 1983 by Society for the Study of Reproduction

ARTICLES

Analysis of human uterine luminal fluid proteins following radiolabeling by reductive methylation: comparison of proliferative and secretory phase samples

DT MacLaughlin and GS Richardson

The proteins contained in human uterine luminal washings of proliferative and secretory phase endometria were depleted of albumin by affinity chromatography and labeled with either 3H or 14C by reductive methylation. Samples of human serum from proliferative and secretory phases of the normal menstrual cycle were similarly treated and served as control material. Isotope mixing experiments were performed in which 3H-labeled fluid proteins were combined with 14C- labeled sera or fluid proteins and the mixture analyzed by two- dimensional gel electrophoresis. The labeled proteins were then detected by fluorography and autoradiography of the same gel. Control experiments mixing 3H- and 14C-labeled sera revealed no difference between proliferative and secretory phase samples and no isotope effect of in vitro labeling. Radiolabeled uterine luminal wash proteins, however, did show significant differences when compared to serum. Furthermore, significant differences between proliferative and secretory phase uterine fluid proteins were observed. A cluster of proteins in the pI range of 5.9 to 6.4 pH and in the molecular weight range of 60,000 -- 67,000 daltons appeared to originate from the endometrium. Among these uterine proteins, several seemed to be found only in the secretory phase of the normal menstrual cycle. The data provided evidence to support the hypothesis that the human endometrium secretes proteins that are dependent on the hormones of the menstrual cycle.


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
R. Pilka, V. Noskova, H. Domanski, C. Andersson, S. Hansson, and B. Casslen
Endometrial TIMP-4 mRNA is expressed in the stroma, while TIMP-4 protein accumulates in the epithelium and is released to the uterine fluid
Mol. Hum. Reprod., August 1, 2006; 12(8): 497 - 503.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the Society for the Study of Reproduction.