Biology of Reproduction, Vol 29, 173-179, Copyright © 1983 by Society for the Study of Reproduction

ARTICLES

In vitro maturation and fertilization of bovine oocytes are temperature- dependent processes

RW Lenz, GD Ball, ML Leibfried, RL Ax and NL First

Effects of temperature on bovine sperm acrosome reaction, oocyte maturation, hyaluronic acid production by cumulus cells and in vitro fertilization were studied. Viability and a true acrosome reaction of bovine spermatozoa were impaired at 40 degrees C. Temperatures lower than 35 degrees C did not enhance the acrosome reaction. However, viability between 30 degrees C-38 degrees C was not altered after 22 h of incubation. The optimal temperature for the acrosome reaction was 38 degrees C. Labeled glucosamine incorporation into glycosaminoglycans was not different among temperatures of 35 degrees C, 37 degrees C or 39 degrees C, whereas 41 degrees C caused a significant reduction (P less than 0.02). Temperatures ranging between 35 degrees C-39 degrees C had no deleterious effects on resumption and completion of meiosis, but at 41 degrees C the frequency of oocytes that progressed to Metaphase II was significantly reduced (P less than 0.0001). Ova matured at 39 degrees C had significantly higher rates of fertilization than at 35 degrees C, 37 degrees C, or 41 degrees C. Killed spermatozoa (control) had no effect on ovum activation at 39 degrees C. From these results it was concluded that events occurring prior to and during fertilization are temperature sensitive.

This article has been cited by other articles:

				G. E. Schrock, A. M. Saxton, F. N. Schrick, and J. L. Edwards Early In Vitro Fertilization Improves Development of Bovine Ova Heat Stressed During In Vitro Maturation J Dairy Sci, September 1, 2007; 90(9): 4297 - 4303. [Abstract] [Full Text] [PDF]

				J Ye, J Coleman, M G Hunter, J Craigon, K H S Campbell, and M R Luck Physiological temperature variants and culture media modify meiotic progression and developmental potential of pig oocytes in vitro Reproduction, May 1, 2007; 133(5): 877 - 886. [Abstract] [Full Text] [PDF]

				C. LaRosa and S. M. Downs Meiotic Induction by Heat Stress in Mouse Oocytes: Involvement of AMP-Activated Protein Kinase and MAPK Family Members Biol Reprod, March 1, 2007; 76(3): 476 - 486. [Abstract] [Full Text] [PDF]

				J. L. Edwards, A. M. Saxton, J. L. Lawrence, R. R. Payton, and J. R. Dunlap Exposure to a Physiologically Relevant Elevated Temperature Hastens In Vitro Maturation in Bovine Oocytes J Dairy Sci, December 1, 2005; 88(12): 4326 - 4333. [Abstract] [Full Text] [PDF]

				R. R. Payton, R. Romar, P. Coy, A. M. Saxton, J. L. Lawrence, and J. L. Edwards Susceptibility of Bovine Germinal Vesicle-Stage Oocytes from Antral Follicles to Direct Effects of Heat Stress In Vitro Biol Reprod, October 1, 2004; 71(4): 1303 - 1308. [Abstract] [Full Text] [PDF]

				R. Sartori, R. Sartor-Bergfelt, S. A. Mertens, J. N. Guenther, J. J. Parrish, and M. C. Wiltbank Fertilization and Early Embryonic Development in Heifers and Lactating Cows in Summer and Lactating and Dry Cows in Winter J Dairy Sci, November 1, 2002; 85(11): 2803 - 2812. [Abstract] [Full Text] [PDF]