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Biology of Reproduction, Vol 13, 38-44, Copyright © 1975 by Society for the Study of Reproduction
1 Department of Zoology, University of Wisconsin,
Madison, Wisconsin 53706 The reprodutive performance of PMSG-primed immature female rats (8 IU PMSG at 30 days of
age) was studied to determine whether they are suitable models or substitutes for adult animals in
studies of reproduction. Pseudopregnancy, induced by cervical stimulation on day 33, lasted 12
days as indicated by leukocytic vaginal smears. Decidual cell reaction was elicited by uterine
trauma on day 5 of pseudopregnancy, and resulted in a fifteen-fold increase in the mean weight of
the traumatized horn within 4 days. PMSG-treated immature rats can conceive, maintain viable fetuses to term, and deliver live pups
in a normal mannner (mean length of gestation: 24.2 days; mean litter size: 7.1 pups). Serum levels
of progesterone, 20 Implantation was delayed in PMSG-primed pregnant rats by bilateral ovariectomy on day 3 and
the injection of 2 mg progesterone daily. Implantation was induced by an injection of 1 µg estrone
on day 10. There was an average of 8.2 implantation sites per animal on day 15, seven days after
the induction of implantation. The postpartum reproductive performance of PMSG-treated mothers and their offspring was
also studied. The growth curves for first and second litters born to PMSG-primed immature rats
were not different from litters born to untreated adult rats. Both first and second litters were
maintained to adulthood. When males and females from the same litters (i.e., first or second) were
mated at random, their breeding performance was comparable to normal adult rats.
-dihydroprogesterone (20-DHP), LH, and FSH were measured by radioimmunoassay in samples taken at 3-day intervals between days 6 and 21 of pregnancy.
Progesterone decreased from 86.7 ng/ml on day 6 to 69.7 ng/ml on day 9, was elevated to 111.4
ng/ml on day 12, and decreased to 64.4 ng/ml on day 21. 20-DHP increased from 7.2 ng/ml on
day 6 to a peak of 14.5 ng/ml on day 15, and decreased significantly to 5.8 ng/ml on day 18.
Serum LU and FSH did not vary significantly. These hormone profiles are similar to those reported
by others in adult pregnant rats.
Accepted on March 3, 1975
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