Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 12, 516-521, Copyright © 1975 by Society for the Study of Reproduction

Separation of Progonadotropic and Antigonadotropic Activities in Ovine and Equine HCG Antisera

H. H. COLE 1, ROBERT DEWEY 1, I. I. GESCHWIND 1, , and MELANIE CHAPMAN 1

1 Department of Animal Science, University of California, Davis 95616


The antigonadotropic factor (antibodies) was separated from the progonadotropic factors (probodies) in ovine and equine HCG antisera and in a gamma globulin fraction of equine HCG antiserum by passing the sera, or a serum salt fractionation product through a column in which HCG had been covalently coupled to activated Sepharose. The probodies passed through the column whereas the HCG antibodies were bound to the HCG on the column.

Accepted on January 7, 1975






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Copyright © 1975 by the Society for the Study of Reproduction.